Journal: Optics Express
Article Title: Continuous focal translation enhances rate of point-scan volumetric microscopy
doi: 10.1364/OE.27.036241
Figure Lengend Snippet: Trade-offs between imaging speed and fidelity for HeLa cells stained with Syto41 (nucleic acids, green) (a). Full image of bottom plane (depth of −1.25 µm) of volume constructed using 3D-FASTR with crop region outlined in purple. (b). Cropped image at 100% Tvol (11.6s) serves as image quality reference. (c/d). Volume representation of neighboring scanned voxels displayed using the MATLAB function Vol3D developed by Joe Conti [20]. These volumes show the number of scanned neighbors for each voxel position with a shift number of (c). m = 1 vs. (d). m = 7. (e-g). Comparison of scanned voxels and final image quality of images acquired at 50% Tvol (5.8s) for different values of n and m. The left-hand side shows the raw images with unsampled pixels labelled blue. The right-hand side shows the image after interpolation. (h-j). Comparison of scanned voxels and final image quality of images acquired in 25% Tvol (2.9s) for different values of n and m. The left-hand side shows the raw images with unsampled pixels labelled blue. The right-hand side shows the image after interpolation. Orange arrows in (f) and (i) highlight curvature artifacts caused by inadequate sampling at 25% Tvol that is remedied by increasing n from 8 to 35.
Article Snippet: Volume representation of neighboring scanned voxels displayed using the MATLAB function Vol3D developed by Joe Conti [ 20 ].
Techniques: Imaging, Staining, Construct, Comparison, Sampling